The Ladybugs are a group of women with "a fast lane lifestyle and slow motion walk" according to Francine. The group seems to be led by Christie White. During "Not Particularly Desperate Housewives", Francine tries to join the group but is rebuffed until a misunderstanding causes them to believe Francine is having an affair with the valet. They reveal to Francine the true nature of their organization: they cheat on their husbands. Francine receives a ladybug brooch and tries to maintain the fiction of her affair with the valet.
However, Francine does not want to cheat on Stan, nor does she involve the valet in her charade. When Francine refuses to show her lover to the Ladybugs, she asks to leave the organization. The Ladybugs refuse, because they fear Francine could betray their secrets, and they have no secrets on her with which to counter-blackmail her. They tell her they wouldn't want her to wind up like Anne Flemming who was crushed by the shopping carts in the beginning of the episode. The Ladybugs make it apparent that they are able to get to Francine at any time since nearly every woman in Langley Falls is a Ladybug. They prepare to kill Francine at the supermarket parking lot the same way they killed Anne, but she is saved in the nick of time by Linda, who passionately kisses her so that the Ladybugs think that Francine's affair is with a woman. Believing that they have a good enough counter-blackmail they leave her alone with a warning.